Mouse Monocyte chemotactic protein 1 (MCP-1) ELISA Kit | KTE71549

Product Overwiew:

This Mouse Monocyte chemotactic protein 1 (MCP-1) ELISA Kit employs a two-site sandwich ELISA to quantitate MCP-1.

Background:

MCP-1 is one of several cytokine genes clustered on chromosome 11. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and memory T cells but not for neutrophils. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.

Host:

N/A

Sequence:

N/A

Reactivity:

Mouse

Application:

This Mouse Monocyte chemotactic protein 1 (MCP-1) ELISA Kit employs a two-site sandwich ELISA to quantitate MCP-1 in samples. An antibody specific for MCP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMCP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCP-1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Used for:

ELISA

Kit components:

• Mouse Monocyte chemotactic protein 1 microplate
• Mouse Monocyte chemotactic protein 1 standard
• Mouse Monocyte chemotactic protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits:

Mouse Monocyte chemotactic protein 1 (MCP-1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MCP-1. No significant cross-reactivity or interference between Mouse MCP-1 and analogues was observed.

Usage Notes:

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage buffer:

N/A

Storage instructions:

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping:

Gel pack with blue ice.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.