abm | Immortalized Human Alveolar Cells | T0487

abm | Immortalized Human Alveolar Cells | T0487

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Lung

Organ Type:

Airway/Lung

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

30-40 hours

Seeding Density:

7,500 cells/cm10²

Markers:

CK-18, CK-19

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days. After P25, abm recommends adding an additional 5% FBS.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR to quantify transgene expression

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C