abm | Immortalized Human Alveolar Cells | T0487
- SKU:
- T0487
- Availability:
- 5 to 7 Days Shipment
- Size:
- 1x10⁶ cells / 1.0 ml
Description
abm | Immortalized Human Alveolar Cells | T0487
Immortalization Method:
Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen
BioSafety Level:
II
Organism:
Homo sapiens
Species:
Human
Source Organ:
Lung
Organ Type:
Airway/Lung
Growth Properties:
Adherent
Morphology:
Polygonal
Passage Number:
N/A
Population Doubling:
30-40 hours
Seeding Density:
7,500 cells/cm10²
Markers:
CK-18, CK-19
Donor Age:
N/A
Donor Gender:
N/A
Donor Ethnicity:
N/A
Propagation:
The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days. After P25, abm recommends adding an additional 5% FBS.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control:
Real Time PCR to quantify transgene expression
Shipping Condition:
Dry Ice
Storage Condition:
liquid nitrogen or -180C