abm | Immortalized Human Amniotic Fluid Stem Cells - SV40 | T0903

abm | Immortalized Human Amniotic Fluid Stem Cells - SV40 | T0903

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 gene.

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Reproductive

Organ Type:

Reproductive

Growth Properties:

Adherent

Morphology:

Spindle-shaped

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is PriGrow VIII (TM018). To make the completed growth medium, add the following components to the base medium: MSC fetal bovine serum (Gibco) to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C