abm | Immortalized Human Osteoprecursor Cells (OPC1) | T0230

abm | Immortalized Human Osteoprecursor Cells (OPC1) | T0230

Immortalization Method:

Serial passaging and calcium phosphate-mediated transfection with a plasmid carrying SV40 T antigen, activated by human A/D interferon

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Bone

Organ Type:

Skeletal Muscle

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

40-50 hours

Seeding Density:

2,800 cells/cm10²

Markers:

Alkaline phosphatase (ALP), osetocalcin (OC), osteonectin (ON), osteopontin (OP), procollagen-I (ProI), and PTH receptor (PTHR)

Donor Age:

12-13 weeks, fetal

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow VIII, available at abm. to a final concentration of 5%, 750 U/ml human A/D interferon (R&D Systems), Penicillin/Streptomycin Solution to a final concentration of 1%, and 0.2 mg/ml G418 for selection.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

1) RT-PCR analysis for osteoblast markers; 2) ALP staining was utilized to determine presence of ALP

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C