abm | Immortalized Human Retinal Pigment Epithelial Cells | T0571

abm | Immortalized Human Retinal Pigment Epithelial Cells | T0571

Immortalization Method:

Spontaneous immortalization in culture

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Retina

Organ Type:

Eye

Growth Properties:

Adherent

Morphology:

Elongated with tapered ends

Passage Number:

N/A

Population Doubling:

48 - 58 hours

Seeding Density:

30,000 - 50,000 cells/cm10²

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

Grow cells in culture wares coated with the Gelatin Coating Solution (0.1%) (TM063) with the following conditions. The base medium for this cell line is Prigrow IV medium available from abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Immunocytochemistry used to detect markers

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C