abm | Immortalized Rat Retinal Pigment Epithelial Cells (BPEI-1) | T0731

abm | Immortalized Rat Retinal Pigment Epithelial Cells (BPEI-1) | T0731

Immortalization Method:

Spontaneous

BioSafety Level:

II

Organism:

Rattus norvegicus

Species:

Rat

Source Organ:

Eye

Organ Type:

Eye

Growth Properties:

Adherent

Morphology:

Fibroblast

Passage Number:

N/A

Population Doubling:

Approximately 18 hours

Seeding Density:

N/A

Markers:

Cellular retinaldehyde binding protein, cytokeratin

Donor Age:

7 days old

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 20% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Immunofluorescence localization; 2) Phagocytosis assay

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C