abm | Immortalized Human Trophoblast Cells - hTERT (Sw.71) | T0532
- SKU:
- T0532
- Availability:
- 5 to 7 Days Shipment
- Size:
- 1x10⁶ cells / 1.0 ml
Description
abm | Immortalized Human Trophoblast Cells - hTERT (Sw.71) | T0532
Immortalization Method:
Serial passaging and transduction with retroviruses expressing hTERT from the supernatant of pA317-hTERT cell line
BioSafety Level:
II
Organism:
Homo sapiens
Species:
Human
Source Organ:
Placenta
Organ Type:
Reproductive
Growth Properties:
Adherent
Morphology:
Polygonal
Passage Number:
N/A
Population Doubling:
14-24 hours
Seeding Density:
20,000-30,000 cells/cm10²; Split ratio of 1:2 to 1:3
Markers:
Cytokeratin 7 and Vimentin
Donor Age:
7-week old
Donor Gender:
N/A
Donor Ethnicity:
N/A
Propagation:
The base medium for this cell line is Prigrow IV medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10%, L-glutamine at a final concentration of 1%, 10mM HEPES (Sigma), 0.1 mM MEM non-essential amino acids (Sigma), 1 mM sodium pyruvate (TM057), and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control:
1) hTERT activity was analyzed via TRAPeze ELISA Detection Kit; 2) Human Cytokine Array 3.1 and III was used to determine cytokine profile; 3) Immunocytochemistry was utilized for determining the presence of cell markers.
Shipping Condition:
Dry Ice
Storage Condition:
liquid nitrogen or -180C