abm | Immortalized Human Trophoblast Cells - hTERT (Sw.71) | T0532

abm | Immortalized Human Trophoblast Cells - hTERT (Sw.71) | T0532

Immortalization Method:

Serial passaging and transduction with retroviruses expressing hTERT from the supernatant of pA317-hTERT cell line

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Placenta

Organ Type:

Reproductive

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

14-24 hours

Seeding Density:

20,000-30,000 cells/cm10²; Split ratio of 1:2 to 1:3

Markers:

Cytokeratin 7 and Vimentin

Donor Age:

7-week old

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10%, L-glutamine at a final concentration of 1%, 10mM HEPES (Sigma), 0.1 mM MEM non-essential amino acids (Sigma), 1 mM sodium pyruvate (TM057), and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) hTERT activity was analyzed via TRAPeze ELISA Detection Kit; 2) Human Cytokine Array 3.1 and III was used to determine cytokine profile; 3) Immunocytochemistry was utilized for determining the presence of cell markers.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C