abm | Immortalized Mouse Calvarial Mesenchymal Progenitor Cells (iCALs) | T0019
- SKU:
- T0019
- Availability:
- 5 to 7 Days Shipment
- Size:
- 1x10⁶ cells / 1.0 ml
Description
abm | Immortalized Mouse Calvarial Mesenchymal Progenitor Cells (iCALs) | T0019
Immortalization Method:
Serial passaging and infection with retrovirus carrying loxP-flanked SV40 large T antigen
BioSafety Level:
II
Organism:
CD-1 Mouse
Species:
Mouse
Source Organ:
Calvariae
Organ Type:
Skeletal Muscle
Growth Properties:
Adherent
Morphology:
Fibroblast-like
Passage Number:
N/A
Population Doubling:
N/A
Seeding Density:
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers:
CD166, CD73, CD44, CD105
Donor Age:
N/A
Donor Gender:
N/A
Donor Ethnicity:
N/A
Propagation:
The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control:
Immunofluorescent staining for mesenchymal stem cell markers
Shipping Condition:
Dry Ice
Storage Condition:
liquid nitrogen or -180C