abm | Immortalized Mouse Calvarial Mesenchymal Progenitor Cells (iCALs) | T0019

abm | Immortalized Mouse Calvarial Mesenchymal Progenitor Cells (iCALs) | T0019

Immortalization Method:

Serial passaging and infection with retrovirus carrying loxP-flanked SV40 large T antigen

BioSafety Level:

II

Organism:

CD-1 Mouse

Species:

Mouse

Source Organ:

Calvariae

Organ Type:

Skeletal Muscle

Growth Properties:

Adherent

Morphology:

Fibroblast-like

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

CD166, CD73, CD44, CD105

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Immunofluorescent staining for mesenchymal stem cell markers

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C