abm | Immortalized Rat Cochlear Microglia Cells (Mocha) | T0740

abm | Immortalized Rat Cochlear Microglia Cells (Mocha) | T0740

Immortalization Method:

Immortalized with retrovirus containing 12S-E1A gene

BioSafety Level:

II

Organism:

Sprague-Dawley Rat

Species:

Rat

Source Organ:

Cochlea

Organ Type:

Auditory

Growth Properties:

Adherent

Morphology:

Flat, with occasional processes

Passage Number:

N/A

Population Doubling:

Approximately 24 hours

Seeding Density:

N/A

Markers:

Neomycin resistance, TMEM119, CD45, Iba1, CD68, Il1-beta

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) PCR gene array; 2) ELISA; 3) ICC

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C