abm | Immortalized Rat Microglia-SV40 | T0246
Serial passaging and transduction with replication incompetent recombinant lentiviruses carrying SV40 Large T antigen
30,000 cells/cm10²; Recommended split ratio is 1:2
The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10%, 4.5g/L D- Glucose (in addition to 4.5g/L D-glucose already present in Prigrow III) and Penicillin/Streptomycin Solution to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
liquid nitrogen or -180C