Description
abm | ADRM1 Stable Knockout HCT116 (Clone B11) Cell Line | T6162
ADRM1 or Adhesion regulating molecule 1, is a component of the proteosome and ubiquitin receptor that recruits the deubiquitinating enzyme, ubiquitin carboxyl-terminal hydrolase L5. Known to undergo O-linked glycosylation, the dysregulation of ADRM1 has been implicated in carcinogenesis making this protein a possible target for drug studies. ADRM1 stable knockout HCT116 (Clone B11) cell line was generated through CRISPR-Cas 9 directed knock exon 4 within the ADRM1 sequence. Exon 4 was replaced with a puromycin resistance cassette and pBluescriptII, allowing for the selection of successfully transformed cells via puromycin resistance.
Biosafety:
II
Organism:
Human
Source Organ:
Colon
Growth Properties:
Adherent
Morphology:
Epithelial-like
Clones:
N/A
Passage Number:
N/A
Population Doner:
25 - 35 hours
Seeding Density:
30,000 - 50,000 cells/cm2
Markers:
Puromycin
Applications:
For Research Use Only
Doner Gender:
Male
Donor Ethnicity:
N/A
Knockdown Method:
N/A
Induction:
N/A
Overexpression:
N/A
Freeze Thaw:
N/A
Propagation:
The base medium for this cell line is Prigrow III medium available at abm
Preservation:
N/A
Quality Control:
N/A
Tumorgenicn:
N/A
Shipping Conditions:
Dry Ice
Storage Contidions:
-180°C