abm | ADRM1 Stable Knockout HCT116 (Clone B11) Cell Line | T6162

abm | ADRM1 Stable Knockout HCT116 (Clone B11) Cell Line | T6162

ADRM1 or Adhesion regulating molecule 1, is a component of the proteosome and ubiquitin receptor that recruits the deubiquitinating enzyme, ubiquitin carboxyl-terminal hydrolase L5. Known to undergo O-linked glycosylation, the dysregulation of ADRM1 has been implicated in carcinogenesis making this protein a possible target for drug studies. ADRM1 stable knockout HCT116 (Clone B11) cell line was generated through CRISPR-Cas 9 directed knock exon 4 within the ADRM1 sequence. Exon 4 was replaced with a puromycin resistance cassette and pBluescriptII, allowing for the selection of successfully transformed cells via puromycin resistance.

Biosafety:

II

Organism:

Human

Source Organ:

Colon

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

25 - 35 hours

Seeding Density:

30,000 - 50,000 cells/cm²

Markers:

Puromycin

Applications:

For Research Use Only

Doner Gender:

Male

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

N/A

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C