abm | KtyI Stable Knockout Mouse Keratinocyte Cell Line | T6293

abm | KtyI Stable Knockout Mouse Keratinocyte Cell Line | T6293

KtyI Stable Knockout Mouse Keratinocyte Cell Line was derived from mice lacking keratins and was spontaneously immortalized. The lack of keratins (ktyl) have been found to reduce desmosome stability and intercellular adhesive strength; compromises cell and tissue integrity. Keratin expression may be re-expressed by the cells vial lentiviral gene transfer to study the affects of keratin alongside with the KtyI Stable Expressing Mouse Keratinocyte Cell Line (T6294).

Biosafety:

II

Organism:

Mouse

Source Organ:

Skin

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

30,000 cells/cm²

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is DMEM/Ham’s F12, low Calcium 50µM) Biochrom). To make the complete growth medium, add the following components to the base medium: chelexed fetal calf serum PAA, A15-151) to a final concentration of 10%, 2mM Glutamax Invitrogen, 35050-038), 1X Pyruvate PAA, S11-003), 0.18mM Adenin Sigma, A8626-5G), 10 ng/ml EGF Invitrogen 53003-018), 2.5 µg/ml insulin Sigma-Aldrich, I-9278), 0.5 µl Hydroxycortison Sigma-Aldrich, H4001-1G), 10^-10 M Cholera toxin Sigma,C-8052), and Penicillin/Streptomycin Solution

Preservation:

1. Freeze Medium: Complete growth medium with 20% chelexed-FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

N/A

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C