abm | Rieske ISP Stable Knockout Mouse Fibroblast Cell Line | T6304

abm | Rieske ISP Stable Knockout Mouse Fibroblast Cell Line | T6304

To generate the Rieske ISP Stable Knockout Mouse Fibroblast Cell Line, primary lung fibroblast culture was established from a knockin mouse homozygous for the floxed UQCRFS1 (exon 2) gene. UQCRFS1 encodes for the Rieske iron sulfur protein, subunit of complex III. Cells were immortalized by retroviral transduction with E6/E7 type 16 papiloma virus carrying G418 resistance. Clones were obtained from immortalization. One of the clones #8 was used to generate the RIESKE KO cells by transfecting with a plasmid expressing cre recombinase. Clones were obtained after hygromycin selection.

Biosafety:

II

Organism:

Mouse (M. musculus)

Source Organ:

Lung

Growth Properties:

Adherent

Morphology:

Fibroblast

Clones:

N/A

Passage Number:

N/A

Population Doner:

24 hours

Seeding Density:

N/A

Markers:

G418 and hygromycin-resistant

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Western blot

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C