abm | c-Kit-WT Stable Ba-F3 (with GNNK- isoform) Cell Line | T3041

abm | c-Kit-WT Stable Ba-F3 (with GNNK- isoform) Cell Line | T3041

c-Kit or stem cell factor (SCF) receptor is a type III tyrosine kinase receptor that has a major role in the hematopoietic system. The c-Kit wild-type-expressing Ba/F3 cells (with GNNK- isoform) are ideal in studies in relation to c-Kit function, mechanisms, and biological activities that may lead to diseases. The GNNK- isoform is the outcome of the c-Kit gene alternative splicing sites, resulting in different isoforms of the tetrapeptide GNNK, and is the main transcript found in human malignancies. Signaling molecules Cbl, Shc, Gab2, Erk and Akt are more strongly activated by the presence of the GNNK- isoform. These cells will proliferate in the presence of the ligand SCF and activate downstream signal transduction. Rapid degradation of the SCF is observed in cells expressing the GNNK-, as the Src inhibitor SU6656 (SUGEN) exerts stronger effects when compared with GNNK+ isoform cells.

Biosafety:

I

Organism:

Mouse

Source Organ:

Marrow

Growth Properties:

Suspension

Morphology:

Round

Clones:

N/A

Passage Number:

N/A

Population Doner:

15 - 25 hours

Seeding Density:

50,000 - 150,000 cells/mL

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Transfected with pMSCVpuro vector carrying wild type human c-Kit construct

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm

Preservation:

N/A

Quality Control:

1) Immunoprecipitation and western blotting for presence of c-Kit and isoform GNNK-; 2) Cell survival assay with stem cell factor (SCF)

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C