Description
abm | CD4 and CCR5 Stable Expressing HEK293T Cell Line | T6332
CD4 and CCR5 receptors are targets for HIV studies. HEK293T cells were transduced with retrovirus carrying pLPCX encoding for human CD4 and CCR5 genes, and were stably selected with the presence of puromycin. CD4 and CCR5 Stable Expressing HEK293T Cell Line is a valuable tool to study the receptors and their function with HIV infection.
Biosafety:
II
Organism:
Human (H. sapiens)
Source Organ:
Kidney
Growth Properties:
Adherent
Morphology:
Epithelial
Clones:
N/A
Passage Number:
N/A
Population Doner:
24 - 34 hours
Seeding Density:
20,000 - 40,000 cells/cm2
Markers:
Puromycin-resistance, human CD4, human CCR5
Applications:
For Research Use Only
Doner Gender:
N/A
Donor Ethnicity:
N/A
Knockdown Method:
N/A
Induction:
N/A
Overexpression:
N/A
Freeze Thaw:
N/A
Propagation:
The base medium for this cell line is Prigrow III medium available at abm
Preservation:
1. Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control:
1) FACS
Tumorgenicn:
N/A
Shipping Conditions:
Dry Ice
Storage Contidions:
-180°C