abm | CD4 and CCR5 Stable Expressing HEK293T Cell Line | T6332

abm | CD4 and CCR5 Stable Expressing HEK293T Cell Line | T6332

CD4 and CCR5 receptors are targets for HIV studies. HEK293T cells were transduced with retrovirus carrying pLPCX encoding for human CD4 and CCR5 genes, and were stably selected with the presence of puromycin. CD4 and CCR5 Stable Expressing HEK293T Cell Line is a valuable tool to study the receptors and their function with HIV infection.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

24 - 34 hours

Seeding Density:

20,000 - 40,000 cells/cm²

Markers:

Puromycin-resistance, human CD4, human CCR5

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) FACS

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C