EBOLA VIRUS NUCLEOPROTEIN (NP) (SUDAN)

Ebola virus Nucleoprotein (Sudan) is a recombinant Ebola virus nucleoprotein expressed and purified from E. coli.

PRODUCT DETAILS – EBOLA VIRUS NUCLEOPROTEIN (NP)(SUDAN)

• Ebola virus (Sudan) Nucleoprotein C-terminus, expressed and purified from E. coli.
• Greater than 95% purity by SDS-PAGE and presented in phosphate buffered saline with 0.02% sodium azide.
• For immunoassay with high specificity and sensitivity.

BACKGROUND

Ebola and Marburg viruses are both filamentous, enveloped, non-segmented, negative-stranded RNA viruses of the family Filoviridae in the order Mononegavirales (Curran and Kolakofsky, 1999; Sanchez et al., 2001). Ebola virus particles comprise seven structural proteins of which four (nucleoprotein (NP), VP35, VP30, and the RNA-dependent RNA polymerase (L)) are components of the ribonucleoprotein complex that is responsible for viral genome replication. Glycoprotein (GP), VP40, and VP24 are membrane-associated proteins and VP24 is believed to be involved in nucleocapsid formation.

Ebola virus NP is the largest nucleoprotein of the non-segmented negative-stranded RNA viruses (739 amino acid residues) and can be divided into a N-terminal hydrophobic region (approximately 350 amino acids) and a C-terminal hydrophilic region (Watanabe et al., 2006). O glycosylation and sialylation of NP is required for its interaction with VP35, and may be important for viral genome replication. The Zaire Ebola virus NP RNA complex has been described at near-atomic resolution using C-terminally truncated protein (residues 1−450) in a human cell line in which it sequestered cellular RNA and assembled it into a rigid helix indistinguishable from the viral nucleocapsid core (Sugita et al., 2018).

Despite the fact that severe hemorrhagic fever caused by Ebola virus is associated with extremely high mortality rates in human and nonhuman primates, an effective vaccine or antiviral drug is still to be developed. This Ebola Virus Nucleoprotein (NP) (Sudan) has been developed to aid in the ongoing research efforts towards developing new vaccine candidates and in vitro diagnostics.

REFERENCES

• Curran and Kolakofsky (1999). Replication of paramyxoviruses. Adv Virus Res. 54:403-22.
• Sanchez et al. (2001). Filoviridae: Marburg and Ebola viruses, p. 1279-1304. In D. M. Knipe and P. M. Howley (ed.), Fields virology. Lippincott-Raven, Philadelphia, Pa.
• Watanabe et al. (2006). Functional Mapping of the Nucleoprotein of Ebola Virus. J Virol. 80(8): 3743–3751.
• Sugita et al. (2018). Cryo-EM structure of the Ebola virus nucleoprotein–RNA complex at 3.6 Å resolution. Nature 563, p.137–140.