Human Apolipoprotein AII (Apo AII) | ABMC-P05

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Human Apolipoprotein AII (Apo AII) | ABMC-P05

Concentration:	1 mg / ml, determined by the Lowry method
Source:	From fresh human plasma that has tested negative for Hepatitis C, HIV-I and HIV-II antibodies as well as Hepatitis surface antigens.
Purification:	After series ultracentrifugations, High Density Lipoprotein (HDL) is isolated from human plasma. Apo AII is purified from delipidated HDL, followed by gel-filtration and DEAE-Sepharcyl chromatography.
Purity:	≥ 98% by SDS-PAGE
Buffer:	20 mM Tris-HCl, 140 mM NaCl, 0.02% NaN3, 0.5 mM EDTA, pH 7.4.
Storage:	-20°C for long-term storage, 4°C for short- term storage. Aliquot to avoid repeated freezing and thawing.

IMPORTANCE

Apo AII comprises 25% of HDL. It exists in human plasma as a dimer of 2 identical chains of 77 amino acid residues, joined by disulfide. The molecular weight is reported to be 8.7 kDa for a single chain (Brewer et al., 1972).

Studies on mouse reported that apo AII may be proatherogenic (Warden et al., 1993); however, case-control study in the large European Prospective Investigation demonstrated that plasma Apo AII concentrations were strongly inversely correlated with CHD events (Birjmohun et al., 2007).

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