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MICROSYPH TPHA200 is a rapid assay for the detection of specific antibodies to Treponema pallidum in human serum or plasma (either di-potassium EDTA, sodium citrate or lithium heparin) by indirect haemagglutination. The kit may also be used for semi-quantitative titration of positive samples.

The 200-test kit contains Control Cells which can be used to assess whether haemagglutination is due to non-specific reactants. This aspect of the kit must not be considered as a confirmatory test for syphilis.

The 1000-test kit is intended to be used as an initial screening test.

Part Number: FTPHA200/1000

Method: Haemagglutination

Description: Test cells, control cells, Diluent, positive and negative controls

  • Convenient and specific test for treponemal infection
  • Minimal equipment required
  • Simple to perform
  • Easily adaptable to automated liquid handling systems for improved throughput

Assay Principle

MICROSYPH TPHA200/TPHA1000 detects human (serum/plasma) antibodies to T. pallidum by means of an indirect haemagglutination (IHA) method.  Preserved avian erythrocytes are coated with antigenic components of pathogenic T. pallidum (Nichol’s strain).  These Test Cells agglutinate in the presence of specific antibodies to T. pallidum, and show characteristic patterns in microwell plates.

Antibodies to non-pathogenic treponemes are absorbed by an extract of Reiter’s treponemes included in the cell suspension. Test results are obtained in 60 minutes and the cell agglutination patterns are easily read and stable.

To facilitate the necessary dilution step a blue dye has been added to the Diluent. This changes colour when the sample is added.

In the 200-test kit, any non-specific reactions occurring are detected using the Control Cells which are avian erythrocytes not coated with T. pallidum.

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1 Review

  • 5


    Posted by Bryson on 6th Jan 2022

    They provide excellent service and deal with their customers in an effective manner.

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