Anti-Type II Collagen (CB8) Antibody, Clone 5A4B11

Anti-Type II Collagen (CB8) Antibody, Clone 5A4B11 - Cat Number: 7065 From Chondrex.

Research Field: Arthrhtis, ECM

Clonality: Monoclonal

Cross-Reactivity: B, C, Ms

Host Origin: Mouse

Applications: ELISA, IHC, WB

Isotype: IgG2a

Detection Range: N/A

Sample Type: N/A

Concentration: 1 mg/ml

Immunogen: Type II Collagen (CB8)

PRODUCT SPECIFICATIONS

DESCRIPTION: Mouse Anti-Type Chick II Collagen (CB8) IgG2a Monoclonal Antibody, Clone 5A4B11,
unconjugated.
APPLICATION: Use for tissue staining, immunoblotting, and ELISA
QUANTITY: 0.1 ml, 1 mg/ml
FORM: Solution in 0.05M Phosphate Buffered Saline, pH 7.4
SOURCE: Mouse
REACTIVITY: Cross-reacts to chick, bovine, and mouse type II collagen.
Epitope: cyanogen bromide-cleaved peptide 8 (CB8) of chick type II collagen (a 149-residue
peptide representing residues 402 - 551 of type II collagen).
IMMUNOGEN: Native chick type II collagen
PURITY: Purified by Protein G chromatography
SUBTYPE: Mouse IgG2a
USAGE: Dilute the antibody in buffer containing 2% heterologous serum, such as normal goat serum or
normal rabbit serum, to prevent non-specific reactions in ELISA and tissue staining. It is
important to use the same species of serum as that of the secondary antibody. Heterologous
serum will be the most effective blocking agent to prevent non-specific reactions. Working
concentration: 0.1 - 1 µg/ml. If detecting denatured collagen, a 10X higher concentration of
cocktail will be required for immunoblotting due to low reactivity to denatured collagen.
STORAGE: -20⁰C
STABILITY: 1 year
NOTES: N/A
REFERENCES: E. Miller, Isolation and characterization of the cyanogen bromide peptides from the l(II) chain of
chick cartilage collagen. Biochemistry 10, 3030-5 (1971).
Hutamekalin. et al. J. Immunol. Method. 343: 49-55 (2009)