Anti-Type II Collagen (CB10) Antibody, Clone C2B-14

Anti-Type II Collagen (CB10) Antibody, Clone C2B-14 - Cat Number: 7116 From Chondrex.

Research Field: Arthritis, ECM

Clonality: Monoclonal

Cross-Reactivity:

Host Origin: Mouse

Applications: ELISA, WB

Isotype: IgG2b

Detection Range: N/A

Sample Type: N/A

Concentration: 1 mg/ml

Immunogen: Bovine Type II Collagen

PRODUCT SPECIFICATIONS

DESCRIPTION: Mouse monoclonal antibody against cyanogen bromide cleaved peptide fragment 10 (CB10) of
type II collagen, clone C2B-14, unconjugated (1).
APPLICATION: Use for tissue staining, immunoblotting, and ELISA
QUANTITY: 0.1 ml, 1 mg/ml
FORM: Solution in 0.05M Phosphate Buffered Saline, pH 7.4
SOURCE: Mouse
REACTIVITY: Cross-reacts to bovine and mouse type II collagen.
IMMUNOGEN: Bovine type II collagen
PURITY: Purified by Protein G chromatography
SUBTYPE: Mouse IgG2b
USAGE: Dilute the antibody in buffer containing 2% heterologous serum, such as normal goat serum or
normal rabbit serum, to prevent non-specific reactions in ELISA and tissue staining. It is
important to use the same species of serum as that of the secondary antibody. Heterologous
serum will be the most effective blocking agent to prevent non-specific reactions. Working
concentration: 0.1 - 1 µg/ml. If detecting denatured collagen, a 10X higher concentration of
cocktail will be required for immunoblotting due to low reactivity to denatured collagen.
STORAGE: -20⁰C
STABILITY: 1 year
NOTES: N/A
REFERENCES: E. Miller, Isolation and characterization of the cyanogen bromide peptides from the l(II) chain
of chick cartilage collagen. Biochemistry 10, 3030-5 (1971).