i7 Hot Start High-Fidelity DNA Polymerase 2X Master Mix

i7^® Hot Start High-Fidelity DNA Polymerase 2x master mix is ready to use premix which contains hot start high-fidelity DNA Polymerase, dNTPs, MgCl₂, PCR enhancers and stabilizers with optimized proprietary reaction buffer.  i7™ Hot Start High-Fidelity DNA Polymerase 2x master mix has the high-fidelity, sensitivity and processivity with an error rate ~2.8×10² fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes.

Description

i7^® Hot Start High-Fidelity DNA Polymerase 2X Master Mix

Intact Genomics (IG) Hot Start i7^® High-Fidelity DNA Polymerase 2x Master Mix is ready to use premix which contains hot start i7^® high-fidelity DNA polymerase, dNTPs, MgCl2, PCR enhancers and stabilizers with optimized proprietary reaction buffer. Hot Start i7 High-Fidelity DNA Polymerase is a chemically engineered, heat stable DNA polymerase which has 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This enzyme has high-fidelity, sensitivity and processivity with an error rate ~2.8×10^2-fold lower than Taq DNA polymerase, and significantly lower than other proofreading enzymes in the marketplace (1). This 2x master mix product is supplied with 5x magic enhancer that enables efficient amplification of GC rich templates up to 84%.

Activity Data
We have tested Hot start i7^® High-Fidelity 2x Master Mix with difficult templates for PCR amplification.

Applications
• Long and difficult template DNA amplification
• Cloning
• High-fidelity PCR
• Efficient for amplifying high GC content template DNA with magic enhancer.

Product Includes
• Hot Start i7^® High-Gidelity DNA Polymerase 2x Master Mix
• 5x magic enhancer

Storage Temperature
-20ºC

Heat Inactivation
No

Storage Buffer
50 mM Tris-HCl, 50 mM KCl,