Intact Genomics propriety ig-Fusion™ cloning technology is a simple, rapid and highly efficient cloning kit which allows to directly clone any PCR product(s) to any linearized expression vector at any site. The PCR fragments can be generated by Intact Genomics high fidelity Pfu DNA polymerase or other high fidelity DNA polymerases, with primers having 15 to 18 bases of homology at their linear ends to where the product need to fuse. The linearized vector can be generated by PCR or restriction enzymes. The kit is so robust that multiple DNA fragments can be assembled simultaneously and cloned into one construct in a single reaction step within short times (usually 10-30 min) with more than 95% cloning efficiency.
- Clone any insert at any site within any vector
- Restriction enzyme and phosphatase free system
- Joining multiple large fragments at once
- Precise insertion at a desired orientation
- Rapid and high efficiency with > 95% positive clones
- 5x ig-Fusion™ enzyme premix
5x ig-Fusion™ enzyme premix: -20 ºC.