HDV RT-PCR Qual | V3-100FRT
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HDV RT-PCR Qual | V3-100FRT from Sacace Biotechnologies is available for delivery
General information: Real Time PCR Test for detection of HDV
Target Disease Type: HDV Associated Kits
Specific Application: Hepatitis D (HDV)
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Hepatitis D, also referred to as Hepatitis D virus (HDV) and classified as Hepatitis delta virus, is a disease caused by a small circular RNA virus. HDV is considered to be a subviral satellite because it can propagate only in the presence of the Hepatitis B virus (HBV). Transmission of HDV can occur either via simultaneous infection with HBV (coinfection) or via infection of an individual previously infected with HBV (superinfection).
Both superinfection and coinfection with HDV results in more severe complications compared to infection with HBV alone. These complications include a greater likelihood of experiencing liver failure in acute infections and a rapid progression to liver cirrhosis, with an increased chance of developing liver cancer in chronic infections. In combination with hepatitis B virus, hepatitis D has the highest mortality rate of all the hepatitis infections of 20%.
HDV Real-TM is a Real-Time test for the Qualitative detection of Hepatitis D Virus in human plasma and simultaneous detection of a HDV-specific Internal Control (IC), by dual color detection.
PRINCIPLE OF ASSAY
HDV Real-TM Test is based on three major processes: isolation of virus RNA from specimens, one-step reverse transcription of the RNA and Real Time amplification of the cDNA. HDV detection by the polymerase chain reaction (PCR) is based on the amplification of pathogen genome specific region using specific primers and detection via fluorescent dyes. These dyes are linked with probes of oligonucleotides which bind specifically to the amplified product. The real-time PCR monitoring of fluorescence intensities allows the accumulating product detection without reopening of reaction tubes after the PCR run. HDV Real-TM PCR kit is a qualitative test which contain the Internal Control (IC). It must be used in the isolation procedure in order to control the process of each individual sample extraction and serves also to identify possible reaction inhibition.
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