Influenza A B RT-PCR (CE) | V36-FRT

Influenza A B RT-PCR (CE) | V36-FRT from Sacace Biotechnologies is available for delivery

Real Time Amplification test for the detection of Influenza A and B Viruses

Storage & Shipping :

The kit can be shipped at 2-8°C for 3-4 days but should be stored at 2-8°C and -20°C immediately on receipt.

Influenza A B RT-PCR (CE) V36-FRT DataSheet

INTRODUCTION

Influenza virus infection, one of the most common infectious disease, is a highly contagious airborne disease that causes an acute febrile illness and results in variable degrees of systemic symptoms, ranging from mild fatigue to respiratory failure and death. These symptoms contribute to significant loss of workdays, human suffering, mortality, and significant morbidity. Influenza results from infection with 1 of 3 basic types of influenza virus – A, B, or C – which are classified within the family Orthomyxoviridae. These single stranded RNA viruses are structurally and biologically similar but vary antigenically. The most common prevailing influenza A subtypes that infect humans are H1N1 and H3N2.

INTENDED USE

Influenza Virus A B Real-TM is Real-Time amplification test for the qualitative detection of Influenza A and B RNA in clinical specimens.

PRINCIPLE OF ASSAY

Influenza Virus A B Real-TM Test is based on three major processes: isolation of virus RNA from specimens, reverse transcription of the RNA, Real Time amplification of the cDNA. Influenza virus A&B detection by the polymerase chain reaction (PCR) is based on the amplification of pathogen genome specific region using specific primers and detection via fluorescent dyes. These dyes are linked with probes of oligonucleotides which bind specifically to the amplified product. The real-time PCR monitoring of fluorescence intensities allows the accumulating product detection without reopening of reaction tubes after the PCR run. Influenza Virus A B Real-TM PCR kit is a qualitative test which contain the Internal Control (IC). It must be used in the isolation procedure in order to control the process of each individual sample extraction and serves also to identify possible reaction inhibition.