Rubella RT-PCR Qual (CE) | V24-50FRT

Rubella RT-PCR Qual (CE) | V24-50FRT from Sacace Biotechnologies is available for delivery

Real-Time PCR test for qualitative detection of Rubella Virus

Storage & Shipping :

Must be stored at 2-8°C.The kit can be shipped at 2-8°C but should be stored at 2-8°C immediately on receipt. .

Rubella RT-PCR Qual (CE) V24-50FRT DataSheet

INTENDED USE

RUBELLA Real-TM Qual is a Real-Time test for the qualitative detection of Rubella (Rosolia) Virus RNA in the plasma, serum, umbilical blood, mucosal swabs (nasal, oral), lavages, amniotic liquid, tissue. RUBELLA RNA is extracted from specimens, amplified using RT-amplification and detected using fluorescent reporter dye probes specific for Rubella or Rubella IC.

PRINCIPLE OF ASSAY

Rubella virus detection by the polymerase chain reaction (PCR) is based on the amplification of the pathogen genome specific region by using specific primers. In real-time PCR, the amplified product is detected using fluorescent dyes. These dyes are linked to oligonucleotide probes that bind specifically to the amplified product. The real-time monitoring of the fluorescence intensities during the real-time PCR allows the detection of accumulating product without re-opening of the reaction tubes after the PCR run. RUBELLA Real-TM Qual PCR kit is a qualitative test that contains the Internal Control (IC), which must be used in the extraction procedure in order to control the extraction process of each individual sample and to identify possible reaction inhibition. RUBELLA Real-TM Qual PCR kit uses “hot-start”, which greatly reduces the frequency of nonspecifically primed reactions. “Hot-start” is guaranteed by separation of nucleotides and Taq-polymerase by using a chemically modified polymerase (TaqF), which is activated by heating at 95 °C for 15 min.