Peptide sequence specific to Zaire Ebola virus (ZEBOV) L-Polymerase protein.
Description:
Affinity purified rabbit polyclonal antibody reactive to Zaire Ebola virus L-Polymerase. The antibody detects L-Polymerase by Western blot in ZEBOV infected HeLa cells.
Supplied:
500 µg of affinity purified antibody is provided in PBS at a concentration of 0.93 mg/mL. 0.01% Sodium azide has been added.
Purification:
Antibody is affinity purified using immobilized immunogen.
Relevance:
The antibody can be used for detection of ZEBOV L-Polymerase
Storage:
2-3 weeks +4oC, -20◦C long term. Avoid multiple freeze thaws.
ELISA:
Not Tested.
WB:
Western blot detection was performed to determine the cross-reactivity of the antibody to virus like particles (VLPs) of Zaire and Sudan ebola virus (ZEBOV and SEBOV) and Marburg virus (MARV) expressing glycoprotein, nucleoprotein and VP40. Reactivity to a ZEBOV (Mayinga) ebolavirus infected HeLa cells and uninfected HeLa cells were also evaluated by Western Blot. Cell lysates were prepared in PBS and lysed with RIPA buffer plus protease inhibitors.
