Description
Capture PR3-ANCA | CAPPR3IU
The Wieslab® Capture PR3-ANCA test kit is an ELISA for detection and quantitation of IgG antibodies to proteinase 3 (PR3) in human sera.
The assay is used to detect antibodies in a single serum specimen and the results of the assay are to be used as an aid to the diagnosis of Wegener’s granulomatosis. It is not intended for screening a healthy population.
Wieslab Capture PR3-ANCA detects antibodies to clinically relevant epitopes with high sensitivity and specificity and is calibrated against the AF-CDC international standard, code IS2721 (Human Reference Serum 16).
What's in the box?
- One frame with 96 wells
- 1.5 mL Negative control (NC) containing human serum in diluent
- 1.5 mL Positive control (PC) containing human serum in diluent
- 13 mL Conjugate
- 2 x 32 mL Diluent (Dil)
- 13 mL Substrate pNPP
- 13 mL Stop solution
- 30 mL Wash solution 30x concentrated
- Six calibrators (five calibrators, Cal 2-6, containing human serum) in diluent. 1.5 mL Cal 1 = 0 IU/mL, 1.5 mL Cal 2 = 2 IU/mL, 1.5 mL Cal 3 = 10 IU/mL, 1.5 mL Cal 4 = 30 IU/mL, 1.5 mL Cal 5 = 100 IU/mL, 1.5 mL Cal 6 = 200 IU/mL
All reagents in the kit are ready for use except wash solution and should be stored at 2-8°C.