Exo-spin™ mini-HD | EX05-5

Size exclusion chromatography (SEC) columns are a reliable method for exosome isolation and can be used as a stand-alone product or as part of a combined strategy for isolating small EVs.

The EX05 Exo-spin™ kit contains packed and equilibrated ready-to-use SEC columns. 

Table 1. The maximum sample size that can be loaded onto a column will depend on the sample type and whether the sample is concentrated by precipitation or loaded iteratively. 
 

Why should I consider Exo-spin™ mini-HD?

The EX05 Exo-spin™ kit has been developed to purify exosomes from intermediate volumes, complementing the range of exosome isolation solutions.

• High-resolution protocol to optimize yield and purity and enable finer fraction collections

• Robust, simple, quick, and economical

• SEC method for excellent exosome recovery

Exo-spin™ column and resin pore size

The column bed volume is 3.5 ml, allowing for 150 µl of volume to be loaded on top of the column.

The pore size of the resin is approximatively 30 nm to attain a highly pure exosome elution. All other molecules (e.g. proteins, lipids) which are smaller than 30 nm will enter into the pores and remain trapped in the column.

Fractionation analysis

The Exo-spin™ mini-HD columns allow a high-resolution fractionation protocol operated by gravity, no special equipment is required.

Highest recovery and purity

As mentioned above, essentially all proteins and lipids will be retained in the column and will elute later than the exosomes, ensuring a highly pure sample ready for your downstream application. A concentration step might be needed prior to SEC isolation depending on your sample type, we offer a precipitation buffer that can be purchased separately (cat code EX06).

Reproducibility

All our columns are manufactured in our laboratory to ensure a high reproducibility between each lot. As proof of reproducibility and batch-to-batch consistency, a large number of peer-reviewed scientific papers have been published describing the use of Exo-spin™.

Storage

Upon receipt, store purification columns at 4°C.

Frequently Asked Questions (FAQs)

For any additional questions, please refer to FAQs document below.

Start today - Select our starter pack

We designed the ideal starter pack to guide your exosome research. The starter pack includes the exosome purification kit of your choice, exosome validated antibodies, and NTA profiling analysis.