Biomedica DKK1 ELISA Highlights:

• Direct measurement - no sample pre-dilution
• Day test

In a first step, assay buffer, standard/control/sample and detection antibody (biotinylated polyclonal rabbit anti-human DKK-1) are pipetted into the wells of the microtiter strips, which are pre-coated with monoclonal mouse anti-human DKK-1 antibody. DKK-1 present in the standard/control/sample binds to the pre-coated antibody in the well and forms a sandwich with the detection antibody. In the washing step all non-specific unbound material is removed. In a next step, the conjugate (streptavidin-HRP) is pipetted into the wells and reacts with the detection antibody. After another washing step, the substrate (TMB, tetramethylbenzidine) is pipetted into the wells. The enzyme-catalyzed color change of the substrate is directly proportional to the amount of DKK-1 present in the sample. This color change is detectable with a standard microtiter plate reader. A dose response curve of the absorbance (optical density, OD at 450 nm) versus standard concentration is generated using the values obtained from the standard. The concentration of DKK-1 in the sample is determined directly from the dose response curve.

DKK1 ELISA Typical Standard Curve

The figure below shows a typical standard curve for the Dickkopf-1 ELISA. The immunoassay is calibrated against recombinant human DKK1.

DKK1 ELISA Kit Components