Human IgA ELISA Kit | F165

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SKU:
214-F165
Size:
1 Kit
Product Type:
ELISA Kit
Storage:
All kit reagents 2°C to 8°C
Assay format:
96 Well Plate
Time to result:
~2 hrs. 50 min
LOD:
150 pg/mL
LLOQ:
~250 pg/mL
Recommended Diluent:
I028
  • Human IgA ELISA Kit
  • Human IgA Kit
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Description

Human IgA ELISA Kit | F165

Human IgA ELISA Kit is intended for use in quantifying very low concentrations of human immunoglobulin A (hIgA). The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. The hIgA assay is a two-site immunoenzymetric assay. Samples containing hIgA are reacted in microtiter strips coated with an affinity purified capture antibody. A second, horseradish peroxidase (HRP) enzyme labeled anti-hIgA antibody, is reacted simultaneously resulting in the formation of a sandwich complex of solid phase antibody-hIgA-enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethylbenzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of hIgA present.

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