MicroVue C3a Plus EIA | A032

The MicroVue C3a Plus EIA measures the amount of C3a-desArg present in human plasma and serum.

C3a is generated during the activation of the complement system via the classical or alternative pathway. The anaphylatoxin C3a itself is very short lived and in serum is cleaved rapidly to the more stable C3a-desArg. Therefore, quantitation of C3a-desArg allows reliable conclusions about the level of complement activation in a test sample. For convenience, we refer to both forms as C3a. The MicroVue C3a Plus EIA measures concentrations of C3a in human plasma and sera. It uses a proprietary monoclonal antibody to a neo-epitope on C3a to capture C3a in the solid phase. The trapped C3a is subsequently detected by HRP-labeled antibodies to C3a antigens. This test provides a rapid, non-radioactive method for assessing C3a levels. It is designed for investigations into the role and or status of complement activation in numerous research and clinical settings. An EIA for the detection of C3a (C3a-desArg) in human serum and plasma.

Kit Format

• Size : 96 wells / test
• Form : 96-well microplate composed of 12 removable 8-well strips, supplied in a resealable foil pouch

Sample Requirements

• Specimen Type : Serum or EDTA plasma
• Sample Volume : 10 µL

Analytical Performance

• Limit of Detection (LOD) : 0.012 ng/mL
• Lower Limit of Quantification (LLOQ) : 0.023 ng/mL
• Upper Limit of Quantification (ULOQ) : 2.531 ng/mL

Precision

• Intra-Assay CV : 4.5–5.3%
• Inter-Assay CV : 5.9–19.6%

Calibration & Controls

• Standards : 5
• Controls : 2

Expected Sample Values

• Serum : 71.0–589.2 ng/mL
• EDTA Plasma : 33.8–268.1 ng/mL

Assay Conditions

• Assay Time: Approximately 2.5 hours

Cross-Reactivity

• African Green Monkey
• Cynomolgus Monkey
• Rhesus Monkey

Storage

• Store unopened kits at 2°C to 8°C
• Refer to the Product Insert for detailed storage and stability information