• Protocols for urine, cell culture and non-human samples
• Extensively validated according to FDA/ICH/EMEA guideline

NT-proCNP ELISA Product overview

The NT-proCNP ELISA kit is a 4 hour, 96-well sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of NT-proCNP in human serum, plasma, urine, cell culture supernatants and non-human samples (rat, mouse, pig).

NT-proCNP ELISA Assay Principle

The CNP ELISA is a sandwich enzyme immunoassay for the quantitative determination of NT-proCNP in human serum, plasma, urine, cell culture supernatants and non-human samples (rat, mouse, pig).

The figure below explains the principle of a sandwich ELISA:

In a first step, assay buffer and standard/control/sample are pipetted into the wells of the microtiter strips, which are pre-coated with polyclonal sheep anti-NT-proCNP antibody, for a short incubation. Without the need of a washing step, conjugate (sheep anti-human NT-proCNP-HRP) is added into the wells. NT proCNP present in the standrad/sample/control binds to the precoated antibody in the well and forms a sandwich with the detection antibody. In the washing step all non-specific unbound material is removed. After a final washing step, the substrate (TMB, tetramethylbenzidine) is pipetted into the wells. The enzyme-catalyzed color change of the substrate is directly proportional to the amount of NT pro CNP present in the sample. This color change is detectable with a standard microtiter plate ELISA reader.

The NT-proCNP ELISA kit uses highly purified, epitope-mapped antibodies. The antibodies utilized in the NT proCNP ELISA (BI-20892) are as follows:

- Capture antibody: polyclonal sheep anti-NT-proCNP (epitope: AA62-68 of Uniprot ID P23582)

- Detection antibody: polyclonal sheep anti-NT-proANP (epitope: AA34-37 of Uniprot ID P23582)

NT-proCNP ELISA Typical Standard Curve

The figure below shows a typical standard curve for the NT pro CNP ELISA. The immunoassay is calibrated against recombinant NT-proCNP peptide:

NT-proCNP ELISA Kit Components