NT-proANP ELISA Assay Principle

This NT pro ANP ELISA kit is a sandwich enzyme immunoassay for the quantitative determination of NT proANP in human serum, EDTA plasma, heparın plasma, cell culture supernatants and urine. The NT-proANP ELISA has also been validated for rat samples.

The figure below explains the principle of a sandwich ELISA:

In a first step, sample and conjugate (sheep anti-human NT-proANP-HRP) are pipetted into the wells of the microtiter strips, which are pre-coated with polyclonal sheep anti-NT-proANP antibody. NT-proANP present in the sample binds to the pre-coated antibody in the well and forms a sandwich with the conjugate. In the washing step all non-specific unbound material is removed. In a second step, the substrate (TMB Tetramethylbenzidine) is pipetted into the wells. The enzyme catalyzed color change of the substrate is directly proportional to the amount of NT-proANP present in the sample. This color change is detectable with a standard microtiter plate ELISA reader.

The NT-proANP ELISA kit uses highly purified, epitope-mapped antibodies.  The antibodies utilized in the NT pro ANP ELISA (BI-20892) are as follows:

Capture antibody: AA 1-30 (polyclonal sheep anti-human NT-proANP 1-98)
Detection antibody: AA 79-98 (HRP-labeled polyclonal sheep anti-human NT-proANP 1-98)