Zymolyase®
, produced by a submerged culture of Arthrobacter luteus, has strong lytic
activity against living yeast cell walls to produce protoplast or spheroplast of various
strains of yeast cells. An essential enzyme for the lytic activity of Zymolyase® is β-1,3-glucan
laminaripentaohydrolase. It hydrolyzes linear glucose polymers with β-1,3-linkages and
releases specifi cally laminaripentaose as the main and minimum product unit There are two preparations of Zymolyase®
, Zymolyase®
-20T and Zymolyase®
-100T,
having lytic activity of 20,000 units/g and 100,000 units/g respectively. Zymolyase®
-20T is
ammonium sulfate precipitate while Zymolyase®
-100T is a further purified preparation by affinity chromatography. Lytic activity varies depending on yeast strain, growth stage of yeast, or cultural conditions. Further informations related to Zymolyase® can be obtained in the reference section below