Description
ExoIntact™ Exosome isolation kit (10 tests) | S.051010 from Nacalai Tesque is available for delivery.
Affinity selection by the peptide realizes higher purity than that in conventional methods such as ultracentrifugation orpolymer-precipitation that entangles unignorable amount of impurities.
Features
- Extraction of highly pure EVs
- Intact EVs are harvested thanks to the mild elution condition
- Applicable to various downstream experiments
Comparison experiment with conventional methods
Comparsion performance with others
EVs were isolated from serum. Then, isolation kits were compared by relative template ratio from Ct value of qPCR and protein concentration by BCA assay.
ORDERING INFORMATION
qNano: Particle Distribution
EV sample was prepared from one test of ExoIntact, exchanged the buffer to PBS by using centrifugal concentrator.
TEM image
EV sample was prepared from one test of ExoIntact without further operations and sent it to Hanaichi Ultrastructure Research Institute to observe a shape of particles. A spherical particle was observed. Its diameter was about 75 nm and 120 nm. This result supports that ExoIntact successfully isolates EVs without damage.