nNOS:C-Terminal (neuronal Nitric Oxide Synthase) Antibody | 24287

(No reviews yet) Write a Review
SKU:
484-24287
Quantity/Volume:
100 µL
  • nNOS:C-Terminal (neuronal Nitric Oxide Synthase) Antibody | 24287
  • IHC image of a single neuron staining for the C-terminal of nNOS in the rat cortex , along with low magnification of cortex (below left), neurons in the rat dentate gyrus (below middle) and in the striatum (below right). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:10000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen, below middle image with nickel stain.
  • IHC image of a single neuron staining for the C-terminal of nNOS in the rat cortex , along with low magnification of cortex (below left), neurons in the rat dentate gyrus (below middle) and in the striatum (below right). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:10000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen, below middle image with nickel stain.
  • IHC image of a single neuron staining for the C-terminal of nNOS in the rat cortex , along with low magnification of cortex (below left), neurons in the rat dentate gyrus (below middle) and in the striatum (below right). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:10000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen, below middle image with nickel stain.
  • IHC image of a single neuron staining for the C-terminal of nNOS in the rat cortex , along with low magnification of cortex (below left), neurons in the rat dentate gyrus (below middle) and in the striatum (below right). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:10000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen, below middle image with nickel stain.
Select Currency //430.00

 Select your currency from the header

Description

nNOS:C-Terminal (neuronal Nitric Oxide Synthase) Antibody | 24287 | ImmunoStar

The ImmunoStar neuronal nitric oxide synthase C-terminal antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, striatum, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/8,000 – 1/12,000 in PBS/0.3% Triton X-100 – Bn/Av-HRP Technique. By Western blot analysis of brain homogenates the antibody specifically labels a band of approximately 155 kD. Immuno-labeling is completely abolished by pre-adsorption with synthetic human nNOS (1419-1433) at 5 µg per mL of diluted antibody. No cross reactivity with other forms of NOS were observed.

Host: Rabbit

State: Lyophilized Whole Serum

Reacts With: Cat, Chick, Ferret, Guinea Pig, Hamster, Human, Monkey, Mouse, Opossum, Rat, Shrew, Squirrel Monkey, Vole, Zebrafish

Availability: In Stock

Alternate Names: Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; Nitric Oxide Synthase; bNOS; Nitric oxide synthase-brain; N-NOS; Peptidyl-cysteine S-nitrosylase; IHPS1; NC-NOS; nitric oxide synthase 1 (neuronal), anti-nNos:C-Terminal

Gene Symbol: NOS1

RRID: AB_572256

View AllClose

0 Reviews

View AllClose

Related Products

Nitric Oxide Synthase Nitric Oxide Synthase
Choose Options
Quick view

Nitric Oxide Synthase | NOS200-1/NOS200-2

Select Currency //256.00 - Select Currency //417.00
Nitric Oxide Synthase | NOS200-1/NOS200-2 | Cell Technology Description Key Benefits Cell permeable. No wash homogenous assay. Adaptable to High throughput assay platforms . Real time detection of...
306-NOS200-1/306-NOS200-2
nNOS:C-Terminal Peptide Control | 24337
Add to Cart
Quick view

nNOS:C-Terminal Peptide Control | 24337

Select Currency //151.00
nNOS:C-Terminal Peptide Control | 24337 | ImmunoStarPre-adsorption of nNOS (C-terminal) antiserum, diluted according to the antibody specification sheet, with 5 µg/ml nNOS peptide immunogen following...
484-24337