MagSi-cfDNA | MDKT00220096

MagSi-cfDNA is intended for (automated) purification of circulating cell-free DNA from human plasma or serum samples. Processing time for the preparation of 24 samples is about 60 minutes.

The kit requires no phenol/chloroform extraction or alcohol precipitation and eliminates the need for repeated centrifugation, vacuum filtration or column separation. It allows safe handling of potentially infectious samples. The obtained cfDNA can be used directly for downstream applications such as qPCR, or any kind of enzymatic reaction.

Features

• Optimized for (automated) use on PurePrep 24
• Suitable for use with fresh or frozen plasma or serum samples
• Plasma can be collected with various blood collection tubes (Streck Cell-Free DNA BCT, EDTA, Citrate etc.)
• Kit provides reagents for 96 extractions of cfDNA from 2 mL sample
• Scalable for use between 1 and 4 mL sample, support protocol for 10 mL sample volume available
• Typical yield: 0.5 to 4 ng cfDNA per mL of human plasma (but highly variable from donor to donor)
• Following lysis at 56°C all other steps are processed at RT
• Does not require carrier RNA
• Magnetic beads are supplied in an optimized storage buffer for decreased sedimentation time
• Suitable for many enzymatic down-stream applications in particular RT-qPCR and sequencing
• Processing time for 24 samples: ~60 min
• Kit comes complete (no additional alcohols required)