Plant Preservative Mixture (PPM)

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PPM - Plant Preservative Mixture

The Plant Preservative Mixture containes antibiotics for plant tissue culture.

Plant Cell Technologies makes this heat stable preservative or biocide for better plant culture and proliferation.

PPM is used to effectively prevent or reduce microbial contamination in plant tissue culture. The Mixture Medium will not inhibit callus production or seed germination.

Medium used for in vitro seed germination, callus proliferation and callus regeneration.

PPM™ prevents the germination of both bacteria and fungi spores and is less expensive than commonly used antibiotics.

Plant in vitro culture
PPM inhibits airborne contamination, waterborne contamination and contamination introduced from human contact, reduce endogenous contamination.

Despite the most stringent use of sterile techniques, the contamination of plant cell and plant tissue cultures remain a persistent problem that can result in losses ranging from small number of cultures to the loss of whole batches.

1. PPM™ is broad-based and effective against fungi.

2. PPM™ is less expensive than antibiotics, making it affordable for wide and routine use.

3. Since PPM™ targets and inhibits multiple enzymes, the formation of resistant mutants towards PPM™ is very unlikely.

4. PPM™ is heat stable and in general can be autoclaved with media.


Bulk PPM 1 liter bottles can be bought from Gentaur.


Plant germination with PPM

PPM affects key Krebs cycle and electron transport chain enzymes. At optimal doses, Plant Preservative Mixture™,



It is an extremely effective preservative/biocide that does not affect in vitro germination , proliferation and callus regeneration. It only inhibits barterial, mycotical and fungal growth.

Despite the use of sterile techniques, contamination of plant cell and tissue cultures remains a persistent problem that can result in losses ranging from a small number of cultures to the loss of entire batches.

PPM™ prevents the germination of bacteria and fungus spores. It is heat stable and can therefore be autoclaved with commonly used antibiotic culture media.

PPM™ can, and should, be used as a standard ingredient in plant tissue culture media. In addition, it is less expensive comparedM™ has been primarily designed to inhibit contamination from microbes in the air and water or contamination from human contact, it can also – in many cases – be used to reduce endogenous contamination.

The sole producer of PPM™ is Plant Cell Technologies Inc., of which Gentaur Molecular Products is the official distributor for France and Europe. 

If you reside in a country outside of Metropolitan France, please contact our head office in Belgium on +32 16 58 90 45.

The principal researcher involved in the development and production of PPM™ is Dr. Assaf Guri. Mr. Assaf Guri, who received his degree in Genetics, Applied Genetics and Plant Breeding from the Hebrew University of Jerusalem and Michigan State University in the United States. Prior to joining Plant Cell Technology, Inc.,

Assaf worked with the Volcani Agricultural Research Center in Israel, Michigan State University in East Lansing, Michigan, and DNAP in New Jersey.

PPM™ (Plant Preservative Mixture) is available in different formats according to the daily needs of our customers:

Reference Condtionnement Unit Price HT
PCT01 30 mL (test)
                 90,00 €
PCT02 100 mL                155,00 €
PCT03 250 mL                305,00 €
PCT04 500 mL                575,00 €
PCT05 1 L                950,00 €

Gentaur France offers discounts for large quantities beyond the second bottle ordered.

Contact us on 01 43 25 01 50 to request a quote or to request a sample certificate of analysis.

The effect of using PPM (plant preservative mixture) on the development of cauliflower microshoots and the quality of artificial seed produced

a b s t r a c t

The effects of using PPM (plant preservative mixture) (Plant Cell Technologies, purchased from Gentaur) on the growth of cauliflower microshoots were determined.

A negative correlation was found between the concentration of PPM in the liquid medium and the number of microshoots developed: the greater the concentration, the lower microshoot number. The stage of the culture process most suitable for the introduction of PPM was also investigated for Coliflower.

While the use of PPM with blending medium (S23: 4.4 g L −1 MS + 30 g L −1 sucrose) did not control the later contamination in the culture medium, the use of 0.5 mL L −1 of PPM with culture medium (S23 supplemented with 2 mg L −1 (9.29 M) of kinetin + 1 mg L −1 (4.9 M) of IBA (indole butyric acid)) was found to be effective in controlling contamination and keeping the growth capacity of microshoots.

Cauliflower microshoots were encapsulated in sodium alginate as artificial seeds. Artificial seeds conversion rate and viability assessed as fresh weights of plantlets produced were evaluated in different culture substrates (compost, perlite, sand and vermiculite).


The effects of PPM concentrations used with S23 irrigation solutions were also evaluated. This study showed the effectiveness of using PPM in controlling the contamination and the necessity for determination the correct concentration and the correct stage for the use of this material in order to obtain optimum results.


PATENT NO. 5,750,402 - The formulation of PPM in tissue culture media at certain concentrations and the use of PPM in
tissue culture at certain concentrations to prevent or eliminate microbial contamination is protected by US patent No. 5,750,402.

Patents have been issued in Canada, New Zealand, Australia, the European Community, Israel and other countries. It is also patent pending in many other countries of the world.


Miyazaki J, Tan BH, Errington SG. Eradication of endophytic bacteria via treatment for axillary buds of Petunia hybrida using Plant Preservative Mixture (PPMTM). PCTOC. 2010;102(3):365-372.
Miyazaki J, Tan BH, Errington SG, Kuo JS. Bacterial endophyte in Macropidia fuliginosa: its localisation and eradication from in
vitro cultured basal-stem callus. Aust J Bot. 2011;59(4):363-368.
Lata H, Chandra S, Khan IA, ElSohly MA. Propagation through alginate encapsulation of axillary buds of Cannabis sativa L. —
an important medicinal plant. Phys and Mol Biol of Plants. 2009;15(1):79-86.
Greer SP, Rinehart TA. Dormancy and Germination In Vitro Response of Hydrangea macrophylla and Hydrangea paniculata
Seed to Light, Cold-Treatment and Gibberellic Acid. J. Environ. Hort. 2010;28(1):41–47.
Moghaddam S, et al. Optimization of an Efficient Semi-Solid Culture Protocol for Sterilization and Plant Regeneration of
Centella asiatica (L.) as a Medicinal Herb. Molecules. 2011;16(11): 8981-8991
Çolgecen H, Koca U, Toker G. Infl uence of diff erent sterilization methods on callus initiation and production of pigmented
callus in Arnebia densifl ora Ledeb. Turk J Biol. 2011; 35:513-520
Pouvreau J, et al. A high-throughput seed germination assay for root parasitic plants. Plant Methods 2013;9:32
Marecik R, Bialas W, Cyplik P, Lawniczak L, Chrzanowski L. Phytoremediation Potential of Three Wetland Plant Species
Toward Atrazine in Environmentally Relevant Concentrations. Pol. J. Environ. Stud. 2012;21(3):697-702
Pérez Flores J, Aguilar Vega ME, Roca Tripepi R. Assays for the in vitro establishment of Swietenia macrophylla and Cedrela
odorata. Rev. Colomb. Biotecnol. 2012;14(1)
Nesterenko-Malkovskaya A, Kirzhner F, Zimmels Y, Armon R. Eichhornia crassipes capability to remove naphthalene from
wastewater in the absence of bacteria. Chemosphere. 2012;87(10):1186-1191.
Kieffer M, Fuller MP. In Vitro Propagation of Cauliflower Using Curd Microexplants. Meth Mol Biol. 2013;994:329-339.
Kodym A, Temsch E, Bunn E, Delpratt J. Ploidy stability of somatic embryo-derived plants in two ecological keystone sedge
species (Lepidosperma laterale and L. concavum, Cyperaceae). Aust J Bot. 2012;60(5):396-404.
Peña-Ramírez YJ, et al. Induction of somatic embryogenesis and plant regeneration in the tropical timber tree Spanish red cedar
[Cedrela odorata L. (Meliaceae)]. PCTOC. 2011;105(2):203-209.
Haddadi F, Adb Aziz M, Saleh G. Abd Rashid A, Kamaladini H. Micropropagation of Strawberry cv. Camarosa: Prolific Shoot
Regeneration from In Vitro Shoot Tips Using Thidiazuron with N6-benzylamino-purine. HortScience. 2010;45(3):453-456.
Jimenez VM, Castillo J, Tavares E, Guevara E, Montiel M. In vitro propagation of the neotropical giant bamboo, Guadua
angustifolia Kunth, through axillary shoot proliferation. PCTOC. 2006;86:389–395.
Compton ME, Koch JM. Influence of Plant Preservative Mixture (PPM) on adventitous organogenesis in Melon, Petunia, and
Tobacco. In Vitro Cell. Dev. Biol.- Plant. 2001;37:259-261
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6 Reviews

  • 4
    Best meristem propagation medium for plant stems.

    Posted by Andreas on 14th Nov 2023

    Meristemic propagation of plants Institute.

  • 5
    Active ingredients

    Posted by Dubois G on 12th Apr 2024

    The active ingredients are 5-chloro-2-methyl3(2H)-isothiazolone and 2-methyl-3(2H)- isothiazolone. That is why for callus in vitro culture in labs the PPM is effective against bacteria and fungi, is heat stable, and can be autoclaved. You only use some microliters per callus stem in your laboratory.

  • 5

    Posted by Dr.Jessica on 5th Mar 2024

    Genprice's shipping of the Plant Preservative Mixture (PPM) exceeded my expectations. The package arrived promptly and was securely packaged to prevent any damage during transit. Overall, I am highly satisfied with the shipping service provided by Genprice and would not hesitate to recommend them to others seeking reliable and efficient shipping for their products.

  • 5
    Schnelle Lieferung

    Posted by Dorothee on 19th Nov 2022

    War am nächsten Tag in Skopje, Macedonia.

  • 5
    easy use

    Posted by Lieven Gevaert on 31st Oct 2022

    The PPM does not inhibit the in vitro seed germination or the good callus proliferation and callus regeneration. PPM prevents the germination of both bacteria and fungi spores.

  • 5

    Posted by Dr. Assaf Guri. Dr. Assaf Guri on 29th Oct 2022

    We bought PPM PLANT PRESERVATIVE MIXTURE from Gentaur Maxanim the distributor of Plant Cell Technology, Inc. in Europe. They told us that Plant Cell Technology has developed this heat stable preservative biocide, to eliminate all microbial contamination in plant tissue culture. It is indead an very effective plant medium decontaminantion agent but it does not impair in vitro seed germination, callus proliferation and callus regeneration. So despite the most stringent use of sterile techniques, the contamination of plant cell and plant tissue cultures remained a persistent problem for us that resulted always in losses ranging from small number of cultures to the loss of whole batches. Thanks to PPM we could prevent the germination of both bacteria and fungi spores. It is heat stable and therefore we could autoclaved it with the PPM. Our plant tissue culture mediais now expensive than the harmful antibiotics we used before! So PPM is designed to inhibit airborne contamination, waterborne contamination and contamination introduced from human contact, but for us it reduces endogenous internal contamination. PPM tissue culture protocol we use: 1. Media containing PPM can be dispensed outside the laminar flow hood (LFH) exposed to the ambient air. The plates should be covered soon after agar solidification. In the event that media dispensing is done by a pump, we recommend passing autoclaved hot water through the hoses prior to and after media dispensing. 2. Heat sensitive or heat stable liquid media containing PPM does not need to be sterilized by Millipore filters or autoclaved provided that it will be stored in sterile containers and that the stock solutions are not contaminated. In rich media containing 200 mg/liter or more of amino acids or proteins, it is recommended to filter the media with the PPM. 3. If working in the LFH the utensils (forceps or scalpels) do not need to be flamed. They should be periodically dipped in 70% alcohol. The LFH does not need to be certified and the work can be done as well outside the LFH on a clean surface for a period not exceeding 1 hour. 4. PPM™ is an acidic liquid solution (pH 3.8). PPM™ should be stored at 4°C. (see Safety Information below). The recommended dose is 0.5 – 2.0 ml of PPM™ per liter of medium is added to the medium before or after autoclavation to prevent airborne and endogenous contamination at low inoculum densities. Higher doses are required to treat endogenous contamination or to obtain Agrobacteria free plant material. 5. PPM™ is less effective when exposed to high density of bacteria or fungi spores found on seed’s coat. For in vitro germination, seeds should be conventionally surface sterilized with bleach. Therefore, in the presence of PPM™ (in the germination medium), the seeds can be rinsed under tap water in a non-sterile strainer and left to dry preferably in the LFH. If the utensil ends have touched active bacteria, fungi culture or otherwise suspected of being contaminated, they should be sterilized by autoclaving or by use of an electric heating element. 6. General dosage levels. With the exception of endogenous contamination, the recommended dose range is 0,05% – 0,2%. For callus proliferation, organogenesis and embryogenesis, the recommended range is 0,05%- 0,075%. Add PPM™ to medium pre or post autoclavation to prevent airborne contamination and endogenous contamination at low inoculum densities or slow growing bacteria. To eliminate higher endogenous contamination densities, higher doses of PPM™ are needed (see paragraph 7). 7. Endogenous Contamination: (a) For seeds: stir non-sterilized seeds for 8-12 hours in 2% PPM™ solution (v/v) supplemented with full strength basal salts of your routinely used medium and don’t add Tween 20 or pH this solution. Subsequently, without rinsing, transfer to germination medium supplemented with 0,05% – 0,1% PPM™ for herbaceous plants and 0,2% PPM™ for woody plants. Hard coated seeds such as Asparagus, Lupine, Ornamental Palm, Rose etc. should be soaked in water for 2-4 hours prior to the sterilization with PPM. (b) For explants: gently shake / stir 1 cm long explants (or shorter) routinely in bleach solution to remove surface contamination. Rinse with water (can be done under non sterile conditions) and shake / stir for 12 hours in 4-5% v/v PPM™ solution supplemented as above with full MS (or 2X) strength basal salts without pH ing and without Tween 20. Without rinsing, insert into a medium supplemented with 0,05% – 0,1% PPM™ for herbaceous plants and 0,2% for woody plants If possible, collect woody plant material after dormancy is broken. This will substantially decrease contamination. (c) For tubers: shake /stir the entire tuber routinely in bleach solution. Rinse with water (can be done under non sterile conditions) and slice the tuber (bulb or scale) into thin slices. Shake / stir for 12 – 24 hours in 4-5% PPM™ solution supplemented with full strength (or 2X) basal salts without pH ing and add Tween 20. Without rinsing insert into a medium supplemented with 0,1-0,2% PPM™. 8. In cases that the above protocols don’t yield satisfied results (especially with thick explants or highly infested explants and seeds), we recommend the followings: (a) Shake / stir the explants (or seeds) in water (does not have to be sterile), 1 hour for soft tissues and 2 hours for hard tissues (b) Shake / stir the explants in 50% PPM™ (dilute with sterile water) supplemented with 2X full strength MS basal salts without pH ing and Tween 20 for 10 – 15 minutes. (c) Without rinsing, insert the explants in to the medium. In fungal contamination, the addition of PPM™ to the medium is optional. However in bacterial contamination or mixed contamination, the addition of 0,05-0,2% PPM™ to the medium in the first month is essential. Don’t discard highly oxidized explants, approximately 50% will recover after 4-6 weeks. 9. To decontaminate “in culture” contaminated explants or plants (rescue treatment), the cultures shouldn’t be left visibly contaminated longer than one week. Clean the material mechanically with the aid of a soft brush under stream of tap water. Shale / stir in a 50% PPM™ solution (diluted with sterile water) for 5 – 15 minutes. In bacterial contamination or mixed contamination, we recommend to lower the pH to the range of 2.8 – 3.2 by mixing 1:1 full strength 100%PPM™ with 0,6 g/l citric acid solution (use sterile water). Without rinsing insert into a medium with 0,05-0,2% PPM™ for at least one month and keep the culture away from high light intensities for the first 10 days. As mentioned above, don’t discard oxidized explants. Wait 4 – 6 weeks. In some cases the fungal or the bacterial spores are located deep within the explants beyond PPM™ reach. In those cases after the water soaking period, slice the explants along and then stir / shake in 50% PPM™ for 10 – 15 minutes.

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