Recombinant Bovine Enterokinase HIS (rbEK-His)
- SKU:
- GEN-NRPA19L
- Formulation:
- liquid is1 U / μl rb-EK, 50mM Tris-HCl, 50% glycerol, pH8.0; Lyophilized from 50mM Tris-HCl (pH8.0) enzyme solution
- Mol. Wt.:
- 26.2 kDa (SDS-PAGE 30 kDa)
- Theoretical PI:
- 5.20
- Specific activity:
- ≥40,000 U / mg, 1 mg rb-EK cleave at least 2000mg fusion proteins
- Purity:
- ≥95% (SDS-PAGE)
- A280nm (1 mg / mL):
- 2.06
- Endotoxin:
- ≤5EU / mg
- Resources:
- Escherichia coli (E. coli)
- Storage state:
- -20°C
- Storage period:
- 3 years
Description
Enterokinase (EC 3.4.21.9) is a serine proteolytic enzyme, which can recognize the sequence of ASP ASP Lys (DDDDK) in protein efficiently and specifically, hydrolyzed peptide binding at the c-terminal of lysine (Lys, K), producing cleavage, hydrolyzes trypsinogen into trypsin in the body. Because enterokinase has high specificity and efficient enzymatic cleavage, it is widely used in genetic engineering. Product development. Natural enterokinase consists of a heavy chain of 115kda and a light chain of 35kDa. The heavy chain anchors the cell membrane, and the light chain has full enzymatic catalytic activity. The central region of light chain activity (235A. A., 26.2kda) secreted and expressed by E. coli is more active than that of bovine enterokinase, which is particularly suitable for the enzymatic digestion of the fusion protein of genetic engineering.
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