HRP Anti-SAM Monoclonal Antibody Clone 118-6 | MAH00201

HRP Anti-SAM Monoclonal Antibody Clone 118-6 | MAH00201 | Arthus Biosystems

Product name         

HRP-anti-SAM 1

Catalog Number     

MAH00201-50/100

Description              

Horseradish peroxidase (HRP) conjugated anti-S-adenosylmethionine monoclonal antibody clone 118-6

Specificity                

MAH00201 shows the following reactivities with related compounds: S-Adenosylmethionine: 100%, S-Adenosylhomocysteine: < 1% , Adenosine: < 1%, L-Methionine: < 1%, Methythioadenosine (MTA): < 1%, ADP (adenosine diphosphate): < 1%, ATP (adenosine triphosphate): < 1%

Immunogen            

S- Adenosylmethionine analog Aza-SAM conjugated to KLH.

Properties

Form                                   

Liquid

Storage instructions          

Store at 4°C, -20°C for long term storage

Storage buffer                    

PBS 10mM pH 7.4 (NaCI 150mM), Proclin 0.1%, Glycerol 50%, BSA 10mg/mi

Purity                                 

>95% Purified from mouse ascites fluid by affinity chromatography

Clonality                             

Monoclonal

Clone number                     

118-6

Immunoglobin isotype      

IgG2b

Research Areas                  

• Methylation of biomolecules (DNA, RNA, proteins, hormones, neurotransmitters, etc.)
• One-carbon metabolism
• Signal Transduction
• Metabolism
• Pathways and Processes Cancers
• Arthritis
• Heart diseases
• Neurodegenerative diseases
• Atherosclerosis
• Liver diseases
• Kidney diseases

Applications

The use of MAH00201 in the following tested applications has been tested. The application notes include recommended starting dilutions. Optimal dilutions/concentrations should be determined by the end user. Higher dilution than suggested maybe used in IHC and IF. The product may be used in other not-yet-tested applications.  Refer to Cat # MA00201 for details.

Notes

cELISA: 1/10,000 - 1/100,000 (depending on the amount of coating)

Figure 1:  (Titer of HRP-118-6) The 0.25 pg/ml of SAM coating standard (Cat # ACT00201) was coated on micro-titer wells. The HRP-118-6 was serially diluted and added to the wells. After incubation of about 60 minutes, substrates were added and OD450 was measured.

Figure 2: The standard curve with HRP-118-6 in direct competitive  ELISA using 4-parameter logistic non-linear regression model. The 0.25 pg/ml of SAM coating standard (Cat # ACT00201) was coated on micro-titer wells. Serial dilution of SAM standard (Cat # AST00201) was added along with 1:25,000 diluted HRP-118-6 and was incubated for about 60 minutes. The substrates were then added and OD450 was measured.

Target

Relevance S- Adenosylmethionine is a common co-substrate involved in methyl group transfers. It is made from adenosine triphosphate (ATP) and methionine by methionine adenosyltransferase. Transmethylation, transsulfuration, and aminopropylation are the metabolic pathways that use SAM. Although these anabolic reactions occur throughout the body, most SAM is produced and consumed in the liver.

Cellular localization  

Cytoplasm, nuclear