HRP Anti-SAM Monoclonal Antibody Clone 84-3 | MAH00202

HRP Anti-SAM Monoclonal Antibody Clone 84-3 | MAH00202 | Arthus Biosystems

Product name           

HRP-anti-SAM 2

Catalog Number      

MAH00202-50/100

Description

Horseradish peroxidase (HRP) conjugated anti-S-adenosylmethionine monoclonal antibody clone 84-3.

Specificity             

MAH00202 shows the following reactivities with related compounds: S-Adenosylmethionine: 100%,

S-Adenosylhomocysteine: < 1% ,Adenosine: < 1%, L-Methionine: < 1%, Methythioadenosine (MTA): < 1%, ADP (adenosine diphosphate): < 1%, ATP (adenosine triphosphate): < 1%

Immunogen              

S- Adenosylmethionine analog Aza-SAM conjugated to KLH.

Properties

Form                                   

Liquid

Storage instructions     

Store at 4°C, -20°C for long term storage

Storage buffer                    

PBS 10mM pH 7.4 (NaCI 150mM), Proclin 0.1%, Glycerol 50%, BSA 10mg/mi

Purity                                  

>95% Purified from mouse ascites fluid by affinity chromatography

Clonality                             

Monoclonal

Clone number                     

84-3

Immunoglobin isotype         

IgG2b

Research Areas                   

Methylation of biomolecules (DNA, RNA, proteins, hormones, neurotransmitters, etc.)

• One-carbon metabolism Signal Transduction Metabolism
• Pathways and Processes Cancers
• Arthritis
• Heart diseases
• Neurodegenerative diseases Atherosclerosis
• Liver diseases
• Kidney diseases

Applications

The use of MAH00202 in the following tested applications has been tested. The application notes include recommended starting dilutions. Optimal dilutions/concentrations should be determined by the end user. Higher dilution than suggested maybe used in IHC and IF. The product may be used in other not-yet-tested applications. Refer to Cat # MA00202 for details.

Application  

cELISA  

Notes                                                       

1/5,000 - 1/50,000

(depending on the amount of coating)

HRP-anti-SAM 2

Dilution

Figure 1 Titer of HRP-84-3   The 0.25 pg/ml of SAM  coating standard (Cat#ACT00201) was coated on micro-titer wells. The HRP-84-3 was serially diluted and added to the wells. After incubation of about 60 minutes, substrates were added and OD450 was measured.

Figure 2 The standard curve with HRP-84-3 in direct competitive ELISA using 4-parameter logistic non-linear regression model. The 0.25 pg/ml of SAM coating standard (Cat # ACT00201) was coated on micro-titer wells. Serial dilution of SAM standard (Cat # AST00201) was added along with diluted HRP-84-3 and was incubated for about 60 minutes. The substrates were then added and OD450 was measured.

Target

Relevance S- Adenosylmethionine is a common co-substrate involved in methyl group transfers. It is made from adenosine triphosphate (ATP) and methionine by methionine adenosyltransferase. Transmethylation, transsulfuration, and aminopropylation are the metabolic pathways that use SAM. Although these anabolic reactions occur throughout the body, most SAM is produced and consumed in the liver.