Starter Kit

WHAT IS INSIDE THE PCT STARTER KIT ?

Are you interested in Tissue Culture but are unsure where to start? The PCT Starter Kit is PERFECT chance for you to try out and hone your new skillset!

In this bundle you will receive:

• MS Media with Vitamins: Make 5L of tissue culture media using the kit.
• 100 grams Supreme Tissue-Culture-Grade Agar: Provide the support your plants need.
• 10 ml PPM™: Fight against all kinds of contamination and protect your cultures.
• 2x Squared Culture Vessels: Provide the perfect environment for your tissue culture plants to grow and develop.
• 1 Disposable Syringe: Easily Measure Your Ingredients for the Perfect Tissue Culture Recipe.

ABOUT SQUARED TISSUE CULTURE VESSELS WITH LIDS:

Feature: Lids Included

Capacity: Plant growth field of 6.15 square inches

Shape: squared container

Color: transparent

Measurements: 3" x 3" x 4"

ABOUT SUPREME TISSUE-CULTURE-GRADE AGAR:

ABOUT MS MEDIA WITH VITAMINS:

MURASHIGE & SKOOG MEDIUM  (W/ VITAMINS & CALCIUM CHLORIDE, W/O SUCROSE, AGAR)

 Product Technical Details:

Supports or facilitate plant growth and/or shoot proliferation in two or more plant tissue cultures (both in monocyledons and dicotyledons).

**FORMULA WT. 4.54 GMS/LTR**

 Physical Properties:

Product currently in back order. All purchases will be pre-order until product is received.  

Instructions for Use:

 Dissolve 4.54gms of dehydrated medium in 600ml of distilled or deionized water at room temperature (15-30*C). Rinse media vial with small quantity of distilled water to remove traces of power. Add the desired heat stable supplements prior to autoclaving. Continue stirring until the powder has dissolved. Some times media does not dissolve completely unless the pH is reduced. For these, lower the pH to about 3.0 to facilitate dissolution of media. The pH of medium is adjusted by using 1N HCL/ 1N NaOH/ 1NKOH. Make up the final volume to 1000ml with distilled water. Mix Gently, heat and rotate between intervals until the solution becomes clear. Do not boil, reheat and allow to cool below 50*C during dispensing. Dispense the medium into suitable containers, plug or cap, then autoclave at 15pso (121*C) for 15 minutes, using a slow exhaust cycle. Higher temperatures and/or longer times are not recommended. Cool the autoclaved culture vessels containing medium to 45-50*C and aseptically add desired sterile heat-labile substrate.

 Note: Media should be prepared according to formula mentioned on the label, however, it is recommended to use an entire container at once. Heat-liable substrates should be added, after autoclaving.

 Storage and Shelf Life:

 Dehydrated plant tissue culture media is hygroscopic and should be protected from sunlight and moisture. Store the prepared medium at 2-8*C away from direct light. Medium should be used before expiry date. The entire volume of each bottle should be used immediately after opening or else the unused portion should be stored in desiccators and refrigerated at 2-8*C.

ABOUT PLANT PRESERVATIVE MIXTURE (PPM™):

PPM™ is a heat stable preservative/biocide which can be used to effectively prevent or reduces microbial contamination in plant tissue culture

• At optimum doses, PPM™, which stands for Plant Preservative Mixture™, is an extremely effective Preservative/Biocide, yet does not impair in vitro seed germination, callus proliferation and callus regeneration.
• PPM™ prevents the germination of both bacteria and fungi spores. It is heat stable and therefore can be autoclaved with the media
• PPM™ can be, and should be used as a standard ingredient in plant tissue culture media, and is also substantially less expensive than commonly used antibiotics

Despite the most stringent use of sterile techniques, the contamination of plant cell and plant tissue cultures remain a persistent problem that can result in losses ranging from small number of cultures to the loss of whole batches.

While PPM™ was principally designed to inhibit airborne contamination, waterborne contamination and contamination introduced from human contact, it can also -- in many cases -- be used to reduce endogenous contamination.