The Cattle QPCR Kit is designed for the target-DNA specific detection of cattle matter in food and animal feed ingredients. The assay uses a real-time PCR-based protocol with fluoresce-probe to detect target DNA. It can detect as few as 10 copies of the target DNA in a reaction and it exhibits high specificity for cattle. No cross reactivity is observed with other animal species (See Table 1). The detection of target DNA confirms ingredient authenticity or prevents food fraud, ethical issues, or health concerns.

Key Features:

• Highly sensitivity and specificity for cattle derived DNA.
• High efficiency: the optimal buffer condition and specific engineered Taq DNA polymerase have increased the efficiency of PCR amplification.
• Streamlined protocol: Just add DNA Template.
• No cross reactivity with other species

Storage Conditions:

The kit is shipped on ice, and stored at -20°C for long-term storage. Shelf life of 12 months after receipt.

PCR Protocol

1. Set up PCR reaction for each sample in 25 µL

Internal control should be included as below: Positive Control (5 µL /reaction), or Negative Control (5 µL/reaction)

2. Suggested PCR conditions