Description
AIV Real-Time Detection Kit is useful for detecting the infection of avian influenza A viruses. The kit can be exactly performed to detect RNA of avian influenza A virus, HS, H7, H9 according to subtype, so it can be used for both qualitative and quantitative analysis. lt works most of Real-Ti me PCR apparatuses of black and capillary type.
Specification
High Sensitivity : Each Real-Time Detection Kit provides highly effective pathogen detection in a sample material. → Possible detection up to 1 O copies/ul.
High Specificity: Real-Time Detection Kit owing optimal& unique primers and probes system guarantees a highly specific result.
Excellent reproducibility: One step Real-Time PCR Kit using Taqman probe.
Quantitative and qualitative analysis.
Complete result within 2 hours for after RNA extraction.
Higher detection limit than general PCR : 100-1,000 times more sensitive.
Simple test procedure: Adding only detection solution and enzyme.
AIV Detection Kits for AIV con tain all component for detection from sample.
Feces, cloaca, and organ homogenates samples are recommended.
Materials in AIV Detection Kit
Contents | Volume |
Standard 1 (1x104 copies/µL) | 200 µLx1 |
Standard 2 (1x105 copies/µL) | 200 µLx1 |
Standard 3 (1x106 copies/µL) | 200 µLx1 |
Standard 4 (1x107 copies/µL) | 200 µLx1 |
Detection Solution* 1 | 460 µLx1 |
Nuclease Free butter | 1500 µLx1 |
2X Enzyme butter | 1000 µLx1 |
Enzyme Mix | 40 µLx1 |
Rox Reference Oye *2 | 40 µLx1 |
Real-Time Detection Kits Procedure
1. Extraction the template RNA
2. Prepare the PCR Mix
3. React the testing sample, Standard 1 ,2,3,4 and negative control at each test tube
4. Set up the cycle program as following the Applicable Real-Time PCR machine
5. Set up the report dye as FAM
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AIV A REAL-TIME DETECTION TEST KIT | PD65-01 | BIONOTE
AIV Real-Time PCR Detection Kit from BIONOTE is useful for detecting the infection of avian influenza A viruses. The kit can be exactly performed to detect RNA of avian influenza A virus, H5, H7, H9 according to subtype, so it can be used for both qualitative and quantitative analysis. It works most of Real-Time PCR apparatuses of block and capillary type.