HbA1c Reagent Kit

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SKU:
FHHBA100
Kit Components:
Reag 1, 1 x 40.8 mL - Reag 2, 1 x 17.1 mL - Lysis Diluent, 4 x 60.0 mL
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Description

INTENDED USE

The HbA1c assay is an Immunoturbidimetric immunoassay for the quantitative determination of percent haemoglobin A1c (HbA1c) in human whole blood on clinical chemistry analysers. Percent HbA1c measurements are used for monitoring long-term glycaemic control in diabetic patients.

HbA1c Reagent Kit DataSheet

SUMMARY

HbA1c is formed by the reaction of glucose with the N-terminal amino group of the haemoglobin beta chain. The Diabetes Control and Complications Trial (DCCT) Research Group previously reported a relationship between percent HbA1c and mean blood glucose levels during the preceding 2-3 months1. The DCCT study also demonstrated that longterm control of diabetes can prevent complications such as cardiovascular disease, retinopathy, nephropathy, and neuropathy. Measurement of percent HbA1c is the method of choice for monitoring therapy of diabetic patients2,3.

PRINCIPLE

The Axis-Shield HbA1c assay utilises the interaction of antigen and antibody to directly determine the concentration of HbA1c (%) in whole blood. Whole blood samples are treated with Lysis Diluent to lyse the red blood cells. Lysed sample is then incubated with latex microparticles (Reag 1). Haemoglobin and HbA1c are captured on the microparticles. When antiHbA1c (mouse monoclonal) antibody (Reag 2) is added, latex-HbA1cantibody complex is formed. The amount of agglutination is measured turbidimetrically and is proportional to the amount of HbA1c absorbed on the surface of the microparticles.

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